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The nasal deformity secondary to cleft lip of 1 0 patients was repaired with lateral segment of partial costal cartilage harvested by a self-made special knife.6 to 30-month follow-up showed that nasal alar morphology of all patients was improved.No any complications happened at donor site.
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Objective:To study the mechanism of the effectiveness loss of methotrexate(MTX)in the treatment of rheumatoid arthri-tis.Methods:The culture system of rat whole bone marrow cells(WBMCs)and tartrateresistant acid phosphatase(TRAP)staining were utilized to evaluate osteoclastogenesis.The mRNA expression of osteoclastogenesis factors in the WBMCs culture system was examined by semi-quantitative RT-PCR.Results:Deoxyadenosine(dAdo)decreased MTX-induced suppression of osteoclastogenesis.The recov-ery effect of dAdo on MTX was partially prevented by caffeine.MTX significantly reduced mRNA expression of receptor activator of nu-clear factor kappa-B ligand(RANKL),dAdo partially recovered RANKL mRNA expression and inhibited osteoprotegerin(OPG)expres-sion.Conclusion:The accumulation of dAdo may induce the effectiveness loss of methotrexate in rheumatoid arthritis treatment.Com-bination of MTX and caffeine can be a potential therapeutic strategy.
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Objective:To study the effects of sulforaphane(SFN)on the proliferation and apoptosis of salivary adenoid cystic carci-noma ACC-Mcells in vitro.Methods:ACC-Mcells were treated with SFN at the doses(μmol/L)of 5,10,20,30,40,60,80 and 100 for 24,48 and 72 hours,respectively.The growth inhibition was examined with MTT assay and typan blue exclusion assay.Morpholo-gy of ACC-M cells was observed with phase contrast microscope,giemsa staining and transmission electron microscope.Flow cytome-try with Annexin-V-FITC/PI double staining was used to detect the apoptosis of ACC-M cells.Results:SFN inhibited the prolifera-tion of ACC-Mcells,the IC50 values(μmol/L)after 24,48 and 72 h treatment were 75.6,21.3 and 16.5 respectively.The highest inhibition rate was 89.2%.The growth inhibition rate of SFN on ACC-Mcells was positively correlated with concentrations of SFN and treatment time.SFN induced the apoptosis of ACC-Mcells in a dose and time dependent manner(P<0.01).Conclusion:SFN can inhibit proliferation and induce apoptosis of salivary adenoid cystic carcinoma ACC-M cells time and dose-dependently.
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Objective:To observe the artifacts produced by dental metal crown made from different materials on magnetic resonance imaging (MRI).Methods:Mental crows made from Co-Gr,Ni-Gr,low-Ti alloy and Pure-Ti were respectively fabricated for upper right second premolar of a ripe crossbreed dog.The 4 different casting crowns were respectively put on experimental tooth and head MRI scan was carried out with 4 sequences of 1.5 T and 6 sequences of 3.0 T MRI respectively.The existence and extent of the arti-facts were assessed and compared.Results:On both 1.5 T and 3.0 T MRI,the Go-Gr alloy crown exhibited significantly wider arti-facts than the others (ANAVO analysis,P0.05).On 3.0 T MRI the artifacts produced by Ni-Gr was larger than that by low-Ti alloy or Pure-Ti(P0.05).By any of the test sequence either on 1.5 T or on 3.0 T,the Co-Gr alloy produced arti-facts in the most slices of MRI imaging and Pure-Ti in the least.Conclusion:Go-Gr alloy may produce larger artifacts than Ni-Gr, low-Ti alloy and Pure-Ti on MRI.
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Objective To introduce experiences with non-elongated cheiloplasty and rectangular mucomuscular complex flaps of double vermilion for repairing bilateral cleft lips. Methods 29 patients (20 males and 9 females) with bilateral cleft lips underwent operations of non-elongated cheiloplasty and rectangular mucomuscular complex flaps of double vermilion. There were 15 bilateral complete cleft lips, 11 bilateral incomplete cleft lips and 3 bilateral mixed cleft lips. The deformity of white lips was reconstructed with traditional non-elongated cheiloplasty. When repairing the vermilion, the rectangular mucomuscular complex flaps which included mucous membrane of vermilion and part of orbicularis oris in double vermilion were designed, and vermilion of fore lip was incised along boundary of wet lip and dry lip. Then rectangular mucomuscular complex flaps of double vermilion were rotated downward and inward to be sewn with fore lip. At the step, the end of orbicularis otis should be sewn widely and fitly in order to form a normal annular structure. Results The wounds of all the patients were healed at one stage. The height and width of patients' upper lips were proper and the center tubercles of the upper lips were obvious. No whistling deformity appeared. Conclusion The method for repairing bilateral cleft lips is simple and reliable. Less tissues could be excised and the satisfactory form and function of nose and lip could be achieved. It is worthy to be applied in clinical practice.
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0.05),that of AR 84.21% and 50.00% (P0.05). Conclusions: The pathogenesis of SS salivary glands appears to be linked to decrease or loss of topical action of androgens.Sex hormones act on epithelial cells of salivary glands through corresponding receptors.
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Objective:To investigate the expression of apoptosis-associated genes bcl-2, bax, fas and fasL in salivary tissue with Sjgrens syndrom(SS). Methods:Bcl-2, Bax, Fas and FasL in salivary tissue from 16 patients with SS and 10 normal controls were detected by SP immunohistochemical staining.Results:Expression of Bcl-2 was significantly decreased(P
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Objective To study the expressions of fragile histidine triad (FHIT) and hMLH_1 in oral squamous cell carcinoma (OSCC) and their correlativity. Methods Immunohistochemistry was used to detect the expressions of FHIT and hMLH_1 in 69 OSCC and 40 normal oral mucosa. The data was analyzed by Chi-square test. Results The positive rate of FHIT and hMLH_1 in OSCC (46.4% and 47.8%) was lower than that in normal oral mucosa (77.5% and 77.5%), with statistically significant difference (P